| 质粒名称: | pcDNA3 RLUC POLIRES FLUC |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 45642 |
| 存储实验室: | Depositing Lab Nahum Sonenberg |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3 |
| 载体出品公司: | Invitrogen |
| 空载体大小: | 5350 bp |
| 完整质粒大小: | 8836bp |
| 载体类型: | 哺乳细胞表达, Luciferase |
| 筛选标记: | 新霉素（G418） |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | RLUC - IRES - FLUC、Renilla luciferase - IRES - Firefly luciferase |
| 插入大小: | 3370bp |
| GenBank编号 | RLUC (1.13.12.5), FLUC (1.13.12.7), PVIRES (M12197) |
| 启动子: | CMV |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | NheI (not destroyed) |
| 3’克隆位点: | XhoI (not destroyed) |
| 5’测序引物 | CMV-F |
| 3’测序引物 | BGH-rev |
|
文献引用方法 |
|
| 材料和方法部分: | pcDNA3 RLUC POLIRES FLUC was a gift from Nahum Sonenberg (Addgene plasmid # 45642 ; http://n2t.net/addgene:45642 ; RRID:Addgene_45642) |
| 参考文献部分: | 4E-BP3, a new member of the eukaryotic initiation factor 4E-binding protein family. Poulin F, Gingras AC, Olsen H, Chevalier S, Sonenberg N. J Biol Chem. 1998 May 29;273(22):14002-7. 10.1074/jbc.273.22.14002 PubMed 9593750 |
买家导航
pcDNA3 RLUC POLIRES FLUC载体质粒基本信息
pcDNA3 RLUC POLIRES FLUC质粒图谱载体图谱和pcDNA3 RLUC POLIRES FLUC载体序列质粒序列多克隆位点信息
pcDNA3 RLUC POLIRES FLUC质粒载体简介
pcDNA3 RLUC POLIRES FLUC质粒序列载体序列
LOCUS pcDNA3 RLUC POLIRES FLUC 8836 bp DNA circular SYN 14-APR-2025
DEFINITION Bicistronic reporter plasmid expressing Renilla luciferase and
firefly luciferase in mammalian cells..
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8836)
AUTHORS Poulin F, Gingras AC, Olsen H, Chevalier S, Sonenberg N
TITLE 4E-BP3, a new member of the eukaryotic initiation factor 4E-binding
protein family.
JOURNAL J Biol Chem. 1998 May 29;273(22):14002-7.
PUBMED 9593750
REFERENCE 2 (bases 1 to 8836)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "J Biol
Chem."; date: "1998-05-29"; volume: "273(22)"; pages: "14002-7"
COMMENT Sequence Label: pcDNA3 RLUC POLIRES FLUC
FEATURES Location/Qualifiers
source 1..8836
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 1..380
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 381..584
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 535..555
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 629..648
/label=T7
/note="T7 promoter, forward primer"
promoter 629..647
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 673..1608
/codon_start=1
/product="luciferase from the anthozoan coelenterate
Renilla reniformis (sea pansy)"
/label=Rluc
/translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
CDS 2420..4072
/codon_start=1
/gene="luc"
/product="firefly luciferase"
/label=luciferase
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
primer_bind complement(2486..2504)
/label=LucNrev
/note="Firefly luciferase, reverse primer"
promoter complement(4155..4173)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(4156..4173)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(4193..4210)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 4199..4423
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 4469..4897
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(4556..4575)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 4766..4787
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(4906..4926)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 4911..5240
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 5091..5226
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 5153..5172
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 5307..6101
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(5361..5380)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 5971..5990
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 6275..6396
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(6312..6331)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 6366..6385
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(6445..6461)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6445..6461)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(6458..6480)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 6469..6485
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(join(6493..6499,6500..6517,6518..6523))
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 6538..6559
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(6676..6693)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6847..7435)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6927..6946)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(join(7606..8397,8398..8466))
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8229..8248
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(8467..8571)
/gene="bla"
/label=AmpR promoter
primer_bind complement(8646..8665)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
ORIGIN
1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
661 gggctagcca ccatgacttc gaaagtttat gatccagaac aaaggaaacg gatgataact
721 ggtccgcagt ggtgggccag atgtaaacaa atgaatgttc ttgattcatt tattaattat
781 tatgattcag aaaaacatgc agaaaatgct gttatttttt tacatggtaa cgcggcctct
841 tcttatttat ggcgacatgt tgtgccacat attgagccag tagcgcggtg tattatacca
901 gaccttattg gtatgggcaa atcaggcaaa tctggtaatg gttcttatag gttacttgat
961 cattacaaat atcttactgc atggtttgaa cttcttaatt taccaaagaa gatcattttt
1021 gtcggccatg attggggtgc ttgtttggca tttcattata gctatgagca tcaagataag
1081 atcaaagcaa tagttcacgc tgaaagtgta gtagatgtga ttgaatcatg ggatgaatgg
1141 cctgatattg aagaagatat tgcgttgatc aaatctgaag aaggagaaaa aatggttttg
1201 gagaataact tcttcgtgga aaccatgttg ccatcaaaaa tcatgagaaa gttagaacca
1261 gaagaatttg cagcatatct tgaaccattc aaagagaaag gtgaagttcg tcgtccaaca
1321 ttatcatggc ctcgtgaaat cccgttagta aaaggtggta aacctgacgt tgtacaaatt
1381 gttaggaatt ataatgctta tctacgtgca agtgatgatt taccaaaaat gtttattgaa
1441 tcggacccag gattcttttc caatgctatt gttgaaggtg ccaagaagtt tcctaatact
1501 gaatttgtca aagtaaaagg tcttcatttt tcgcaagaag atgcacctga tgaaatggga
1561 aaatatatca aatcgttcgt tgagcgagtt ctcaaaaatg aacaataatt ctagagcggc
1621 ccccaagctt gggctgcagg tcttaaaaca gctctggggt tgtacccacc ccagaggccc
1681 acgtggcggc tagtactccg gtattgcggt acctttgtac gcctgtttta tactcccttc
1741 ccccgtaact tagaagcaca atgtccaagt tcaataggag ggggtacaaa ccagtaccac
1801 cacgaacaag cacttctgtt cccccggtga ggctgtatag gctgtttcca cggctaaaag
1861 cggctgatcc gttatccgct catgtacttc gagaagccta gtatcacctt ggaatcttcg
1921 atgcgttgcg ctcaacactc aaccccagag tgtagcttag gtcgatgagt ctggacgttc
1981 ctcaccggcg acggtggtcc aggctgcgtt ggcggcctac ctgtggccca aagccacagg
2041 acgctagttg tgaacaaggt gtgaagagcc tattgagcta cctgagagtc ctccggcccc
2101 tgaatgcggc taatcctaac cacggagcag gcagtggcaa tccagcgacc agcctgtcgt
2161 aacgcgcaag ttcgtggcgg aaccgactac tttgggtgtc cgtgtttcct tttattttta
2221 caatggctgc ttatggtgac aatcattgat tgttatcata aagcaaattg gattggccat
2281 ccggtgagaa tttgattatt aaattactct cttgttggga ttgctccttt gaaatcttgt
2341 gcactcacac ctattggaat tacctcattg ttaagatatc catcacactg gcggccgcat
2401 ctagagggcc cggatccaaa tggaagacgc caaaaacata aagaaaggcc cggcgccatt
2461 ctatcctcta gaggatggaa ccgctggaga gcaactgcat aaggctatga agagatacgc
2521 cctggttcct ggaacaattg cttttacaga tgcacatatc gaggtgaaca tcacgtacgc
2581 ggaatacttc gaaatgtccg ttcggttggc agaagctatg aaacgatatg ggctgaatac
2641 aaatcacaga atcgtcgtat gcagtgaaaa ctctcttcaa ttctttatgc cggtgttggg
2701 cgcgttattt atcggagttg cagttgcgcc cgcgaacgac atttataatg aacgtgaatt
2761 gctcaacagt atgaacattt cgcagcctac cgtagtgttt gtttccaaaa aggggttgca
2821 aaaaattttg aacgtgcaaa aaaaattacc aataatccag aaaattatta tcatggattc
2881 taaaacggat taccagggat ttcagtcgat gtacacgttc gtcacatctc atctacctcc
2941 cggttttaat gaatacgatt ttgtaccaga gtcctttgat cgtgacaaaa caattgcact
3001 gataatgaat tcctctggat ctactgggtt acctaagggt gtggcccttc cgcatagaac
3061 tgcctgcgtc agattctcgc atgccagaga tcctattttt ggcaatcaaa tcattccgga
3121 tactgcgatt ttaagtgttg ttccattcca tcacggtttt ggaatgttta ctacactcgg
3181 atatttgata tgtggatttc gagtcgtctt aatgtataga tttgaagaag agctgttttt
3241 acgatccctt caggattaca aaattcaaag tgcgttgcta gtaccaaccc tattttcatt
3301 cttcgccaaa agcactctga ttgacaaata cgatttatct aatttacacg aaattgcttc
3361 tgggggcgca cctctttcga aagaagtcgg ggaagcggtt gcaaaacgct tccatcttcc
3421 agggatacga caaggatatg ggctcactga gactacatca gctattctga ttacacccga
3481 gggggatgat aaaccgggcg cggtcggtaa agttgttcca ttttttgaag cgaaggttgt
3541 ggatctggat accgggaaaa cgctgggcgt taatcagaga ggcgaattat gtgtcagagg
3601 acctatgatt atgtccggtt atgtaaacaa tccggaagcg accaacgcct tgattgacaa
3661 ggatggatgg ctacattctg gagacatagc ttactgggac gaagacgaac acttcttcat
3721 agttgaccgc ttgaagtctt taattaaata caaaggatat caggtggccc ccgctgaatt
3781 ggaatcgata ttgttacaac accccaacat cttcgacgcg ggcgtggcag gtcttcccga
3841 cgatgacgcc ggtgaacttc ccgccgccgt tgttgttttg gagcacggaa agacgatgac
3901 ggaaaaagag atcgtggatt acgtcgccag tcaagtaaca accgcgaaaa agttgcgcgg
3961 aggagttgtg tttgtggacg aagtaccgaa aggtcttacc ggaaaactcg acgcaagaaa
4021 aatcagagag atcctcataa aggccaagaa gggcggaaag tccaaattgt aaaatgtaac
4081 tgtattcagc gatgacgaaa ttcttagcta ttgtaatcct ccgaggcctc gagcatgcat
4141 ctagagggcc ctattctata gtgtcaccta aatgctagag ctcgctgatc agcctcgact
4201 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
4261 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
4321 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
4381 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
4441 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg
4501 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
4561 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
4621 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
4681 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
4741 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
4801 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
4861 aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt
4921 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
4981 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
5041 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
5101 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
5161 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg
5221 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc
5281 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc
5341 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
5401 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
5461 acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca
5521 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
5581 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
5641 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
5701 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
5761 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
5821 ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
5881 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
5941 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
6001 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
6061 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga
6121 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt
6181 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
6241 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
6301 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
6361 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
6421 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
6481 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
6541 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
6601 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
6661 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
6721 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
6781 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
6841 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
6901 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
6961 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
7021 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
7081 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
7141 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
7201 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
7261 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
7321 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
7381 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
7441 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
7501 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
7561 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
7621 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
7681 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
7741 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
7801 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
7861 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
7921 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
7981 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
8041 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
8101 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
8161 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
8221 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
8281 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
8341 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
8401 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
8461 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
8521 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
8581 ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact
8641 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg
8701 ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc
8761 gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg
8821 gccagatata cgcgtt
//
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pcDNA3 RLUC POLIRES FLUC质粒载体应用举例
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